Multichannel Systems HiClamp Manual - Manual (Page 41)

TEVC Recording Protocol
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The Notebook
An electronic laboratory notebook is built in the HiClamp program. Access to this logbook is
automatic when the first sequence is activated. The notebook window cannot be turned off
during the experiment but can be minimized and recalled by a single click on the corresponding
icon. The notebook window is divided in two parts. The upper section presents the notes that
have been entered during the experiment. These notes are stored in the corresponding *.lab file
in the data folder (see data file structure below). Notes are entered in the lower section of the
window and can be modified as long as they are not entered or automatically stored by the
program. The time for the beginning and ending of the experiment is automatically generated
and stored in the logbook file. For security purposes the logbook is encoded and cannot be edited
with a text processing program. Attempts to edit the logbook should therefore be avoided as they
may destroy the file and your records.
5.7.1 Measuring multiple oocytes
Playing the simple example presented before measures the properties of the first oocyte and
stops. To take advantage of the HiClamp it would be important to be able to measure an entire
set of oocytes. This is readily possible by introducing a loop that will repeat the sequence as many
time as desired. Following the addition of the loop icon the sequence is now the following.
Note that the choice of the Loop with No Increment is indicated by the square arrow. Having
introduced the loop it is now necessary to indicate at which step the program should loop back.
This is readily done by a double click first on the loop icon and the action is indicated by a
change in the pointer from the arrow to a circle. A double click on the target step (in our
case the load plate icon) opens a control window in which the number of times the loop should
be executed is entered (for example 12 times to capture the response of an entire column of the
microtiter plate).
Following the entry (done) the program sequence displays the steps that will be executed with
a blue line in the lower part indicating how the loop will execute and at which steps it returns.
The number of times the loop will be executed is indicated by the number displayed below the
icon loop.