Eppendorf SOP - Manual (Page 35)

35
Adjustment
Eppendorf SOP
English (EN)
7.3.4 Research plus fixed
Adjustment is carried out as described for the Research plus
adjustable-volume(see p. 25).
7.4 Physical influences of liquids
It is possible to adjust the previously described devices for a volume of a liquid
with a different density than water so that the displayed volume value
corresponds to the pipetted volume.
The adjustment process is comparable to the described procedure (see p. 18).
The difference is that the mean value of the weighings is converted to
microliters according to the formula:
.
1. Determine the mean value and convert it to microliters.
The calculated value is the nominal value.
2. The digital display of variable pipettes, or the volume of fix-volume pipettes,
must be set to the calculated actual value.
3. Check the value set for the liquid gravimetrically. The accordingly set device
only delivers a dispensing value, which corresponds to the digital display, for
the used liquid and set volume.
4. If necessary correct and check the setting.
5. Label the measuring device following adjustment with the measured value
and the name of the liquid which was used for the adjustment.
With adjustable-volume pipettes, all other values are then readjusted, as
the adjustable-volume pipette then becomes a fix-volume pipette.
Following adjustment, the certificate accompanying the pipette
becomes invalid.