UVP Doc-It Life Science - Manual (Page 102)

Performing Colony Counting
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Merging Colonies
Users may merge colonies after performing the initial count. Colonies should be merged when
one colony is treated as two separate colonies by the software. Colonies will only be merged if
they are less than 4 pixels apart.
To merge colonies, ensure that the Merge Colonies tab is highlighted in yellow.
A new window will ask the user to Select the desired colonies and then press Merge
button to join them.
Click onto the colonies to be merged.
The Total colonies count will change to reflect the changed due to using the merge
function.
A rectangular object will appear. Move it over the area of interest.
The central sample image will appear larger in the area of interest identified.
If the colony is hard to see, zoom in on the colony.
User Defined Template Counting
Before proceeding through the next steps, a template must have been saved during the Manual Counting
process.
Select Start Colony Count, select User Defined Template Counting, and then
select the desired template from the dropdown menu.
Click OK.
The new window will display the total count.
Spiral Counting
Perform Spiral Count
To perform a spiral plate count:
Select the Spiral checkbox from the Main Tools section of the Colony Count module.
Select Start Colony Count.
Count the colonies.
NOTE: If using the software for the first time, no templates are created and the User
Defined Template Count option appears grey. To create a template, first proceed
through the Manual Count functions to store settings.
Adjust Grid Size and Volume
After performing a count, a Spiral Plate Analysis window will appear along with a green grid
over the counted image.
To change the overlay grid size: