UVP Doc-It Life Science - Manual (Page 54)

Using Image Filters
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Scan an Image from a Scanner
Select Image > Scan > Start Scanning.
Change any settings offered by the device as desired. Consult Help for the scanner to learn
about the Scan dialog window for your device.
Click Scan (this button may have different names, including OK or Acquire, for different devices).
The image will appear in a new Image window inside the workspace.
Tip: Most scanners offer a "fast preview" mode. You can use this to adjust brightness,
contrast and other settings appropriately before scanning.
Spatial Calibration
Using the Image Scale Tool
There are two ways to set the image's scale:
If the size of a single feature anywhere in the image is known, use the Scale Tool to set the scale
of the entire image from that one feature.
If the size of the entire image is known, change the Sample Width through the Image Information
window.
Calibrate the Image Using the Scale Tool
Choose Tools > Spatial Calibration > Define Image Scale.
Find the feature in the image for which the size is known. Click one edge of the feature.
(It may be useful to position a small ruler with the Petri dish and capture the image of the
ruler and Petri dish together so that the ruler can be used for reference)
Move to the other edge of the feature. As the mouse is moved, the scale tool will show
how many pixels are marked with the tool.
Click the other edge of the feature. The Set Scale window will appear.
In the Set Scale window, select the metric unit for the marked area (e.g. centimeters)
and enter the number of units (e.g. 12).
NOTE: The marked area must be selected first and then the number of units second.
Click OK. The rulers will reflect the new scale.
Changing the Sample Width for an Image
Sample Width is calculated from the width of the image. It is the distance of the width of the scene in the
image. For reference, a ruler may be placed across the top of the image so that the entire distance of the
scene can be determined.
Right click onto the image and select Image Information for the image.
There are two tabs present in the Image Information window. Ensure that the
Properties tab is selected.
In the Overall Sample Width area, select the unit measurement (the right-most drop-