UVP Doc-It Life Science - Manual (Page 88)

Performing 1D Analysis
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Molecular Weight Calibration
Calibration of molecular weight (MW) involves associating a known standard with one or more lanes in
the image. This allows Rf values to be calibrated to molecular weight values. LS Software allows the user
to employ several different standards per gel.
To help with analysis, LS provides a library of molecular weight standards. Standards can be added,
edited, and deleted from the library using the following instructions.
Adding a Molecular Weight Standard to the Library
Select the lane to calibrate by clicking on it.
Select Analysis > 1D Analysis > Master Tools > Molecular Weight or select Molecular Weight
in the 1D Analysis Plugin. A new window appears.
In the Step 1: Select MW standard tab, click Add. A new Create MW standard window opens.
In this window, provide the new Name of the standard, Group, and Unit Type.
Click Add. Now the numerical values of the standard can be entered. After entering the numerical
value, click on Add again for as many values as desired
Click OK on the right side. The first window appears again with the new standard entered.
Editing a Molecular Weight Standard in the Library
Select the Lane to calibrate by clicking on it.
Select Analysis > 1D Analysis > Master Tools > Molecular Weight or select Molecular Weight
in the 1D Analysis Plug-in. A new window appears.
In the Step 1: Select MW standard tab select the standard to edit. (NOTE: This applies only to
standards created by users. The MW Standards included with the software cannot be edited or
deleted.) Then click on Edit.
The information previously entered into this window (including group, name, units, or most
commonly, weight) can now be changed.
Click Edit to reflect the new change, and then click OK.
Deleting a Molecular Weight Standard in the Library
Select the Lane to calibrate by clicking on it.
Select Analysis > 1D Analysis > Master Tools > Molecular Weight or select Molecular Weight
in the 1D Analysis Plugin. A new window appears
In the Step 1: Select MW standard tab select the standard to delete. (NOTE: This applies only to
standards created by users. The MW Standards included with the software cannot be edited or
deleted.) Then click on Delete. LS software then confirms that the standard will be deleted. Select
Yes to confirm.
Click on Cancel to return to the image window.
Copying a Molecular Weight Standard in the Library
Select the Lane to calibrate by clicking on it.
Select Analysis > 1D Analysis > Master Tools > Molecular Weight or select Molecular Weight
in the 1D Analysis Plug-in. A new window appears.