UVP Doc-It Life Science - Manual (Page 99)

Performing Colony Counting
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Manual Counting Step 1: Select Classes
The window that appears after choosing Manual Count is the manual count window. It contains
two tabs. The first tab is Step 1 of 2: Select Classes and the second tab is Step 2 of 2: Finish.
Defining the Counting Region
This tab allows the user to define the region of interest. The region of interest is identified by a
green circle that should include all the colonies (or zones) of interest in the Petri dish. The
software automatically selects a region of interest but by clicking the Define Counting Region
button, but the user can increase or decrease the circle size.
If a smaller or larger region of interest is desired, click the Define Counting Region button and
hold and drag the pointer over one of the corners of the circle to change the size.
Select Classes
The Select classes window allows users to select the number of classes and colonies (or
zones) in the Petri dish sample.
To select the desired colonies (or zones), ensure that the Add Points button is
highlighted in yellow and click on a colony (or zone) in the original image of the Petri
dish. (Not the image shown in Step 2 of 2: Select classes window.)
Classes are defined as different types of bacteria, yeast, or mold present on the same
Petri dish sample. The software can detect various types of classes in one dish. To add
a class, click onto the Add button in the Classes section of the window.
Once the first desired colony (or zone) is selected, the new image in the Step 1 of 2: Select
classes window will show a black background along with all of the colonies that contain the
same color as the point selected.
The color of the point selected will also appear in the Analysis Details box (for example: rgb
(202,183,151).
Continue adding points and classes as necessary until the black and white image
shows all the colonies of interest in white.
To remove a class or a point, highlight the class or point in the Analysis Details box and click
the Remove Point button.
NOTE: The Step 1 of 2: Select classes tab allows the settings created in this window to be
saved in a user defined template. If there are no templates created, the drop down menu will list
Default as the only template option.
Manual Counting Step 2: Finish
To filter the colonies (or zones), adjust the shape or colony size (or add and subtract zones) in
the Step 2 of 2: Finish, Filter colonies section:
Change slider buttons for shape (from circular to irregular) and size (1 pixel to 200
pixels) to capture additional colonies or eliminate colonies (or until all of the zones of
interest are highlighted).
This window is a second means to save settings into templates. Go to the Templates