UVP Doc-It Life Science - Manual (Page 83)

Performing 1D Analysis
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Background correction is necessary to account for possible variable illumination or overexposure during
image capturing, LS software offers options to apply mathematical background correction. These options
generally remove background "noise" and elevated levels of pixel intensity due to excess exposure,
highlighting data. There are four corrections options that are explained in the Specific Correction Options
section of this chapter.
From the Analysis > 1D Analysis > Master Tools > Background Correction or the 1D Analysis plugin
dropdown menu a new list appears.
Using the 1D Analysis Plugin
From the list select which type of background correction, if any, that the software should perform.
Viewing Background Correction in the Lane Profile Graph
Choose the Analysis > 1D Analysis > Master Tools > Lane Profile Graph or click on Lane profile
graph from the 1D Analysis Plugin.
On the graph, in the second box below Background Correction, click the down arrow to show the drop-
down menu of the background correction options. Click the appropriate correction type. The graph will
now display the background correction in the selected lane as a dotted line.
Specific Correction Options
LS Software offers background correction options.
No background correction
Straight line
Joined valleys
Area between the lines
Rolling disc
No Background Correction
Selecting this option on the menu leaves the image uncorrected for overexposure, "as is."
Straight Line
Selecting this option tells LS Software to place a straight (but not necessarily horizontal) line under the
lowest points at the beginning and end of each lane. LS removes the area of the graph under the straight
line, so that all remaining values are emphasized. Straight line correction tends to correct well for
overexposure, and for variable illumination that is focused on an edge or corner of an image.
Note: Straighten lines by using right-clicking the mouse button to open the
shortcut menu.
Joined Valleys
Selecting this option accentuates the data by telling LS software to join lines between the lowest point, or
"valley", before the first band, between each pair of bands, and after the last band. Intensities above the
valleys (band data) are emphasized. Joined valleys can perform well in a variable illumination condition
where the "bright spot" is somewhere in the middle of the image, and where bands are sharply defined