UVP Doc-It Life Science - Manual (Page 92)

Performing 1D Analysis
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are red marks on the Rf line created, the Rf line will not be created when Confirm Add is
selected. Ensure that the entire line is green before clicking Confirm Add.
Click Close when finished adding Rf lines.
Moving Rf Lines
Existing Rf lines can be adjusted, whether they were created automatically or manually. To move Rf lines
to match bands of the same molecular weight:
Click Analysis > 1D Analysis > Master Tools > Edit Objects or select Edit objects in the 1D
Analysis Plug-in.
Select the Rf line to adjust. Control handles will appear.
Any lane that has a white control handle without an "X" is an uncalibrated lane, and it is possible
to move the intercept point up or down by dragging it. If the lane has a gray control handle with an
"X," it is already calibrated and cannot be moved.
After Rf lines have been adjusted hide them so other 1D objects can be seen more easily. From
the Analysis menu, select 1D Analysis Settings, then turn off Rf Lines.
Deleting Rf Lines
Rf lines that were added manually can also be removed (automatic Rf lines cannot be removed).
To Remove One Rf Line
Click Analysis > 1D Analysis > Master Tools > Edit Objects or select Edit objects in the 1D
Analysis Plugin.
Select the Rf line to delete in the image.
From the Analysis > 1D Analysis > Rf lines and click Delete selected Rf line(s) or select Delete
selected Rf line(s) from the 1D Analysis Plugin.
Tip: Rf lines can also be deleted by selecting it and pressing the DELETE key.
To Remove All Rf Lines
From the Analysis > 1D Analysis > Rf lines and click Delete all Rf lines or select Delete all Rf
line(s) from the 1D Analysis Plugin.
Performing Dendrogram Analysis

Overview

Dendrogram Calculation

Multi-Image Dendrogram Calculation

Modify Graphical Display