UVP Doc-It Life Science - Manual (Page 82)

Performing 1D Analysis
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The Analysis Bands Report:
Band Name and ID
Calculated peak values (Rf value and molecular weight);
Intensity of the band, including its maximum, volume, percentage of the lane, and mass; and
Concentration of the band, including its maximum, volume, percentage of the lane, and mass.
The Lane Profile Report: Gives a graphical representation of lane data.
To Print the Reports
Under the Tools Menu, select Reports. Select the report needed by clicking into the checkbox. For
example, if the Analysis Settings report was needed, the user would click onto the Analysis Settings
check box.
Select Print, OK. Click Exit to close the Reports window.
To Change General Layout Before Printing
To change the margins, click on the Page Setup then make the margins larger or smaller by varying
degrees. To view the margin lines, select Show Margins in the Preview Mode in the Reports window.
Enter information in Header Text or Footer Text. Note the following abbreviations for header and footer
text:
%p puts the page number at the top or bottom of the page;
%c puts the page count at the top or bottom of the page;
%d puts the date at the top or bottom of the page; and
%t puts the time at the top or bottom of the page.
Performing Concentration Calibrations
Overview
Background Correction
Concentration Calibration
Overview
Having sized and moved lanes and bands, concentration calculations can be performed. This section
explains how to:
Correct background for overexposure or uneven exposure of light or chemicals;
Show the concentration graph for calibration;
Select data points to plot on the graph;
Select the type of curve to fit to the data points; and
Remove all concentration calibration data.