UVP Doc-It Life Science - Manual (Page 55)

Using Image Filters
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down list) first. For example, if the sample width of the image is 500 cm, select
centimeters.
Type the number of metric units in the text box immediately following Overall Sample
Width. For example, if the sample width of the image is 500 cm, type "500."
Click OK.
Using Spatial Calibration for Annotation
Special types of annotations are available from Tools > Spatial Calibration option:
Define Image Scale: Allows the user to spatially calibrate to a known measurement.
Measure Length: Length Measure annotations look somewhat like Line annotations except that
they show the length of the line in image scale units (see "Rulers"). Position Length Measure
annotations from one point to another point to see how long any image feature is in real units.
Select the color, line thickness, line style (solid, dotted or dashed), font size and formatting (bold,
italic or underline) of a Length Measure annotation.
Measure Angle: Angle Measure annotations consist of three points. They display the angle
between the line formed by the first and second points and the line formed by the second and
third points. Use Angle Measure annotations to determine slants in lanes or in related bands
across lanes in an electrophoresis gel. Select the color, line thickness, line style (solid, dotted or
dashed), font size and formatting (bold, italic or underline) of an Angle Measure annotation.
Measure Area: Area Measure annotations look somewhat like Rectangle annotations except that
they show the area of the rectangle in image scale units (see "Rulers"). Select the color, line
thickness, line style (solid, dotted or dashed), font size and formatting (bold, italic or underline) of
an Area Measure annotation.
Note: Length Measure and Area Measure annotations use the image's scale in
calculations. If these annotations display information in pixels ("px"), no scale has
been set. See Changing Sample Width in this chapter for instructions on setting
the image's scale.