UVP Doc-It Life Science User Manual

Page 71

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Performing 1D Analysis

66

Move the cursor over the image. A movable horizontal line will appear whenever the cursor is
moved over a lane. Simply move the mouse over the image to the spot where to place the new
band. If the color of the horizontal line is green, a new band can be placed where the cursor is. If,
however, the color is red, there is already a band at this position and a new band cannot placed
there. Move the mouse until the line appears green or adjust the other bands extents before
adding the band.

Click the left mouse button to place the new band.

When finished placing band(s), click on 1D Analysis > Master Tools > Edit Objects to disable
the Add Bands tool.

Move and Resize Lanes and Bands

LS software allows the user to move and resize lanes and bands.

To Move Lanes or Bands Using the Image Window

Click Analysis > 1D Analysis > Master Tools > Edit Objects or use the 1D Analysis plugin >
Master Tools > Edit Objects.

Select the lane or band to move by clicking on it. Control handles will appear at the four corners
of the lane as white boxes.

Drag the box side to side (or up and down) until it is moved to the desired location. Note that
lanes can only be moved between other lanes, not beyond other lanes. Bands can only be moved
between the bands above and below it, not beyond other bands.

To Move Bands Using the Lane Profile Graph

Click Analysis > 1D Analysis > Master Tools > Lane Profile Graph, or use the 1D Analysis
plug-in > Master Tools > Lane Profile Graph. The graph appears in a new window.

Ensure that Band Peaks and Band Extents are both selected. Under the graph find the band
markers. Drag the peak marker of the band (the large marker in the middle of two smaller
markers) to the desired location for that band. Note that the markers on the graph change
position as well. Note that bands can only be moved between the bands above and below it, not
beyond other bands.

Click on OK. The Lane Profile window closes and bands are now in their new positions.

Note:

When editing bands or lanes, ensure that 1D Analysis > Master Tools > Edit
Objects
is selected prior to clicking on a band or lane.

Resize Band Extents

To Resize Bands using the Image Window

To turn on and view the Band Extents, go to Analysis > 1D Analysis > Settings to open the
Preferences Window. Select the 1D Analysis tab and click the Band Extents check box. Click
Apply. Close the Preferences Window.

Click Edit Objects from the 1D Analysis plugin. Place the mouse hand pointer over the band to
resize, and click on the band. Control handles will appear at the four corners of the band as red
boxes.

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