Image controls plug-in, Image controls – UVP Doc-It Life Science User Manual

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Using Plug-In Modules

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Cancel: Closes the window and cancels the process.

Saving .AVI Files

If files need to be shared with another researcher or opened in another place on another computer export
the .sqv files to a standard universal format. (SQV is a custom format used by UVP LLC).

Audio Video Interleaved (AVI) is one such broadly acceptable format. (It is a simplistic Windows format
that caters to the needs of slow animation, audio and video.)

To import an .AVI file, no special steps are required to be taken since it is one of the standard formats
supported by LS Software.

To export a .SQV file to .AVI, use the Save As functionality provided by UVP Software.

Depending on the requirement of the target player, AVI files may need to be compressed/ encoded using
a specific codec. Before exporting, LS software allows users to choose from one of the pre-installed
codecs.

Note: Use the SQV whenever possible. AVI file types reduce the image to 8-bit.

Image Controls Plug-In

The Image Control plug-in offers features to control how an image looks. Changes made to images using
this plug-in will not be permanent changes to the image. All the changes can be reversed with the Reset
button.

Go to View > Plugins to load the Image Controls plug-in.

Specific features available on the Image Control module are:

Brightness: affects the overall brightness or dimness of the image. Brightness level of 50 means
the image is displayed in its original brightness (i.e. unchanged). Changing the brightness level
can make features near the top or the bottom of the intensity scale easier to see.

Contrast: affects the difference between light and dark parts of the image. A contrast level of 50
means that the image is displayed in its original contrast. A level higher than 50 means that
contrast has been increased (lights are lighter, darks are darker). A level lower than 50 means
that contrast has been decreased (lights and darks are both closer to middle values). Increasing
the contrast tends to highlight differences in intensity level; decreasing it can make patterns that
cross intensities more clear.

Gamma: also affects the difference between light and dark parts of the image, but it does so by
using a "gamma correction curve." The gamma correction curve affects middle values more
quickly than values at either the darkest or the lightest ends of the spectrum. Gamma contrast
values range from 0.1 to 5.0. A value of 1.0 means that no gamma correction curve is in effect
(the image is displayed at its original levels). Gamma contrast changes have similar results to
regular contrast changes.

Invert: reverses all intensities, light for dark and dark for light. This also will have the effect of
complementing colors (e.g. red to turquoise, yellow to blue). Inverting the image can make certain
features easier to see.

Change Brightness, Contrast, Gamma Or Invert

Change Brightness

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