UVP Doc-It Life Science - Manual (Page 90)

Performing 1D Analysis
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Using Manual Placement of Weights
If the user does not want to use the Stretch Factor, the weights can be adjusted individually using manual
mode:
In the Step 2 screen of the Molecular Weight tool, select Independent calibration mode.
Move each weight separately, with either the mouse or the keyboard arrows, to position them
exactly on the band. Weights cannot pass one another, so it is usually best to start with either the
lightest or heaviest weight and work toward the other end.
Exact Placement of Bands
In the Independent calibration mode, as soon as the weight is exactly on the band, the color of the line
changes from blue to yellow (default colors). This means exact placement is achieved-- the weight will be
exactly on the band peak. Exact placement only occurs when the color changes from unmatched to
matched; further movement of the weight may alter the exact positioning. When in doubt, move the
weight completely away from the band and reposition it with the arrow keys until the color changes.
The Independent > Tag All button reduces some manual work by aligning the weights of the ladder
starting with first band in the lane. Stretch factor is not taken into account
– only simple matching is done.
Note: On the second window of the calibration operation (where users adjust weights to
bands), the colors of both the unmatched and the matched lines can be changed by
using the controls in the upper right corner.
Note: After calibration of two or more lanes, Retardation factor lines will be automatically
calculated and will replace any previous Rf line work.
Removing a Molecular Weight Standard from a Lane
If a Molecular Weight (MW) Standard has already been calibrated to a lane, the calibration can be
removed from a lane by doing the following:
Select the Analysis > 1D Analysis > Master Tools > Molecular Weight or select Molecular
Weight from the 1D Analysis Plugin.
A new Molecular Weight window appears. Go to the Step 1: Select Molecular Weight
Standard tab.
Choose Uncalibrate lane.
Click Yes to confirm the removal of the MW calibrated lane.