ChemoMetec SP-100 - Manual (Page 11)

Introduction and intended use
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Introduction and intended use
The NucleoCounter SP-100 is intended for use in connection with production of semen
doses for artificial insemination within the field of animal husbandry.
The NucleoCounter SP-100 is designed to count sperm
cells in semen from mammalian animals
The instrument is part of the NucleoCounter SP-100 system, which also comprises a
disposable SP1-Cassette, Reagent S100 (lysis/dilution buffer) and PBS (dilution buffer).
The NucleoCounter SP-100 system allows reliable, fast and objective cell counting to be
carried out based on an automated method of fluorescence microscopy. The system also
enables the user to stain and count sperm cells without being exposed to potentially
hazardous DNA dyes.
The optimal firmware settings of the NucleoCounter SP-100 vary from species to species.
Semen from most species, e.g. bulls, stallions, dogs and bucks (rabbit), can be tested with
the same instrument settings.
However, semen from Boars requires different instrument settings due to weaker
fluorescense signals from boar sperm cells compared to sperms from most other species.
If the instrument firmware version is 1.23 or lower, the type of species is set from the
factory and this setting cannot be changed by the operator.
If firmware version 1.24 – 1.30 is installed in the instrument, the species can be selected
using the F11 command. However, the F11 command does only affect the settings used for
a Total Count. The settings for the Non-Viable Count is not changed by the F11 command
and therefore the factory settings is being used. Contact ChemoMetec for more
information about changing to the correct Non-Viable settings.
If firmware version 1.31 or higher is installed in the instrument, the species can be
selected using the F11 command. Furthermore, the F11 command will in addition to
selection of the correct settings for a Total Count, also select the correct settings for a
Non-Viable Count.
The NucleoCounter SP-100 is developed as a stand-alone instrument but optionally it
may be connected to a computer via an USB-interface or to a printer via RS-232 printer
port. When connected to a computer the SemenView software offers various features
such as documentation of the obtained results. Read more about the software in the
SemenView User’s Guide.
The NucleoCounter SP-100 part number is 900-0100. Refer to Section 11.1 with respect
to other part numbers of the NucleoCounter SP-100 system.
The NucleoCounter SP-100 system is not intended for human diagnostic purposes.