ChemoMetec SP-100 - Manual (Page 53)

6 Description of a typical Analysis Procedure (Total Count)
35
6 Description of a typical Analysis Procedure
(Total Count)
The Application Notes describe the analysis procedures in detail (e.g. Application Note
No. 100 deals with the analysis of boar ejaculates).
Always refer to the latest version of the appropriate Application Note .
In this section a brief description of Application Note No. 100 Version 1 is given. The
individual procedural steps are illustrated with pictures.
The following list contains the items required for determing sperm cell density in boar
semen with the NucleoCounter SP-100 system.
•
NucleoCounter SP-100 with software version 1.04 or higher
•
SP1-Cassettes
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Container with Reagent S100 (lysis/dilution buffer)
•
20 ml polyethylene sample cups with polypropylene lids
•
Container holder and sample cup holder
•
Bottle-top dispenser 1-10 ml, e.g. 1-10 ml Brand Dispensette III Variabel
dispenser equipped with a 298 mm filling tube
•
Adjustable, automatic pipette, e.g. 10-100 µl Finnpipette adjusted to give 50 µl
boar semen ejaculate, and pipette tips
•
Optionally the NucleoCounter SP-100 can be connected to a computer via an
USB-interface. When connected to a computer the SemenView TM software offers
various features such as documentation of the obtained results.