ChemoMetec SP-100 User Manual
Page 25
1 Basics of the SP-100 system
7
The NucleoCounter SP-100 is equipped with advanced software for image analysis. This
integral software analyzes the recorded image and counts the number of nuclei in the
image. As the volume of the cassette measurement chamber and the dilution factor is
known, the cell density in the initial sample can be calculated. The concentration of
sperm cells in the initial sample (in millions per ml) is presented in the NucleoCounter
SP-100 display. If the instrument is connected to a PC via the USB-interface, the results
and the recorded fluorescent image can be viewed with the SemenView software (Refer
to User’s Guide for SemenView). A printer can also be connected to the RS-232 printer
port of the instrument. However, only the result and the important parameters and not
the fluorescent image can be transferred to the printer. Please refer to
Technical Note
No. 100
with respect to the printer application.
The entire process takes approximately 30 seconds (from pressing “
Run” to presentation
of the result). A Non-Viable Counts takes approximately twice the time of a Total Count
(i.e. 60 seconds) because a more complex analysis is carried out in the instrument.
Performing a cell count with the NucleoCounter SP-100 is objective and virtually
independent of the operator.