ChemoMetec SP-100 - Manual (Page 54)

6 Description of a typical Analysis Procedure (Total Count)
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Briefly, the Total Count analysis procedure comprises the following steps:
1. Type in the Sample ID on the NucleoCounter SP-100 and check that the Dilution
Factor is correct.
2. Using the automatic pipette mounted with a new pipette tip aspirate 50 µl of
the ejaculate. Transfer the sample to the center area of the bottom of a 20 ml
sample cup (see
Figure 63 ).
Figure Figure Figure Figure 63 63 63 63.... Pipetting of 50 µl of sample (A) and transferring sample to sample
cup (B)
3. Place the sample cup in the cup-holder and dispense 10.00 ml of Reagent S100
into the cup using the dispenser (see Figure 64 ). Dispense the reagent directly
onto the semen sample at the bottom of the cup. Use a consistent plunger
pressure, so that the plunger moves smoothly. There is no need for further
mixing, since the sample and the reagent are thoroughly mixed during the
dispensing of the Reagent S100.
Figure Figure Figure Figure 64 64 64 64.... Placing the sample cup in the cup holder (A), dispensing 10 ml of
Reagent S100 (B) and sample cup with lyzate mixture (C)
B A
C B A