ChemoMetec SP-100 User Manual

Page 48

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5 Operation of NucleoCounter SP-100

30

5.16 Viability mode - F31 (only relevant for instruments with Viability Option

included)

If the Viability Option is supplied with the instrument the Viability mode can be
activated by the F31 command:

Display Text Type 29

Viab.mode Off

Viab.mode Off

Viab.mode Off

Viab.mode Off

1:On

1:On

1:On

1:On

2:Off

2:Off

2:Off

2:Off

store

store

store

store

Figure 57. Use the “1” and “2” buttons to toogle between Viability mode set to On or

Off. Press “↵

” to save the chosen setting or press “

Esc” to cancel.

With respect to carrying out a viability measurement (in fact the viability is calculated
from a Total Count and a Non-Viable Count), please refer to

Viability Testing with

NucleoCounter SP-100, Addendum to User’ s Guide

(part no. 991-0104).

5.17 Inserting and removing the Cassette

When a cassette has been properly loaded with lyzate mixture, as explained in

Section

1.1.2

, the cassette is inserted into the NucleoCounter SP-100 instrument.

The grey lid covers the cassette insertion area. This lid is designed to protect the area
from dust and other potential contaminants, and to keep external light from interfering
with the fluorescent image recording. The lid is fitted with magnetic hinges, so it can be
easily removed for cleaning purposes.

To insert the cassette:

1. Flip the lid up

2. Grip the cassette on either side of the piston barrel, but taking care not to

touch the measurement windows (see

Figure 58

).

3. Insert the cassette, bevelled edge down, into the insertion slit

(see

Figure 58

).

After analysis the lid is lifted and the cassette is removed from the insertion slit and
disposed properly according to national or local laws or regulations regarding the nature
of the mixture it contains.

Unless you are inserting/removing a cassette or cleaning the inserting area or the lid,
keep the lid closed at all times.

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