Hach-Lange DR 6000 Basic User Manual User Manual

Page 161

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English 161

Multi Wavelength mode – readings with more than one

wavelength

In the Multi Wavelength mode, absorbance values can be measured at
up to four wavelengths and the results can be mathematically processed
to obtain sums, differences and relationships.

Absorbance readings: The light absorbed by the sample is
measured in absorbance units.

% Transmittance measures the percent of the original light that
passes through the sample and reaches the detector.

Turning on the concentration factor allows selection of a specific
multiplier for converting absorbance readings to concentration. In a
graph of concentration versus the absorbance, the concentration
factor is the slope of the line. Concentration is calculated using a
single factor for each wavelength, which is input by the user.

Set reading mode at different wavelengths

Press Multi Wavelength in the Main Menu. Press Options for
Parameter Setup.

Table 4 Multi wavelength setup options

Options

Description

More

For further Options

Recall
measurement
data symbol

Recall saved measurement data, wavelength scans or time
courses, refer to

Save, recall, send and delete data,

page 154

.

% Trans/Abs

Switches from % transmittance to concentration or
absorbance in reading mode.

λ

Wavelength

To input the wavelength setting. Use the alphanumeric
keypad to enter the reading wavelengths. A wavelength in
the range of 190–1100 nm can be entered.

Timer icon

This functions as a stopwatch. It helps to make sure that the
steps of an analysis are correctly timed (e. g. reaction times,
wait times, etc., can be exactly specified). When the
specified time has elapsed, a sound is emitted. The use of
the timer has no influence on the reading program.

Concentration
Factor

Multiplication factor for converting absorbance values into
concentration values.

Concentration
Resolution

To select the number of decimal places.

Absorbance
Formula

Calculation basis for evaluating samples

Save as User
Program

To store the selected parameters as a User Program, refer to

Single Wavelength (absorbance, concentration and
transmittance readings), page 159

.

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