Eppendorf epMotion 5070 - Manual (Page 30)

Dispensing liquids
epMotion
® 5070
English (EN)
30
6.1.2 Liquid aspiration up to the remaining volume
Abb. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth
Fig. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth
The pipette tip is immersed 3 mm into the liquid before liquid aspiration. The pipette tip moves downward
during liquid aspiration. The immersion depth of 3 mm remains constant while the liquid level decreases.
If the pipette tip reaches a distance of 1 mm to the base of the vessel, the pipette tip reduces its immersion
depth to 0.7 mm. If the liquid level continues to sink, the pipette tip will not aspirate any additional liquid.
The remaining volume remains in the vessel.
A remaining volume is present because the pipette tip has a minimum immersion depth in the liquid and a
minimum distance to the base of the vessel. Under normal conditions, the remaining volume is calculated
using a filling level of 1.7 mm.
The distance of the pipette tip to the base of the vessel can be changed in the software (refer to epMotion
software operating manual).
6.1.3 Bottom tolerance
The bottom tolerance is the distance between the vessel base and the lower end of the pipette tip. The
default setting for the bottom tolerance is 1 mm. For 30 mL and 100 mL reservoirs, the default setting for
the bottom tolerance is 2.5 mm.
Decreasing the bottom tolerance also reduces the remaining volume.
1. Removing liquid above a pellet increases the bottom tolerance.
2. Do not decrease the bottom tolerance on uneven plates.
3. Check the set bottom tolerance when exchanging pipette tips, plates or vessels.
If the liquid is aspirated from the remaining volume, the bend of the liquid level can lead to
dispensing errors.
1 mm
0,7 mm