Eppendorf epMotion 5070 - Manual (Page 33)

Dispensing liquids
epMotion ® 5070
English (EN)
33
The volume for the additional aspriation depends on the dispensing tool; the software will take it into
account when calculating the required aspiration volume.
Example for aspiration volume for multi-dispensing
A 96-well plate should be filled with 10 μL water in each well via multi-dispensing. The eight-channel
dispensing tool TM 50 is used. Aspiration is performed from one reservoir. The tips are not changed before
a new liquid aspiration.
Sum of the aspiration volumes for multi-dispensing:
• 96 x 10 μL for 96-well plate: 960 μL
• 8 x 5.8 μL reverse stroke: 46.4 μL
• 8 x 2.5 μL additional aspiration: 20 μL
• Sum: 1026.4 μL
6.2.3 Mixing
Liquids can be mixed via multiple pipetting.
A mixing cycle consists of one upward movement and one downward movement of the piston in the
dispensing tool.
The aspiration level and dispensing level of the liquid can be defined during mixing. A defined height
should only be used for levels below the working volume as the vessel may overflow with larger levels.
During the mixing process, the pipette tip is in the liquid. The blow-out will be executed above the surface
of the liquid after the mixing procedure.
Defined mixing speeds are defined in the liquid types. When using your own settings, determine the
optimal mixing speed via experiments.
Dispensing tool Additional volume per
channel for reverse stroke Additional volume per
channel for remaining stroke
Single-channel dispensing tool TS 50
Eight-channel dispensing tool TM 50 5.8 μL 2.5 μL
Single-channel dispensing tool TS 300 16.7 μL 3.7 μL
Eight-channel dispensing tool TM 300 45.2 μL 5.0 μL
Single-channel dispensing tool TS 1000
Eight-channel dispensing tool TM 1000 50.3 μL 35.2 μL
NOTICE! Contamination of the dispensing tool due to high mixing frequency.
If the mixing frequency is too high, liquid may enter the dispensing tool.
 If the liquid has a low viscosity or tends to foam, set a low mixing frequency.
 Use demineralized water to test the mixing settings.
 Use pipette tips with filter.