Eppendorf epMotion 5070 - Manual (Page 81)

Troubleshooting
epMotion ® 5070
English (EN)
81
11.2.2 Dispensing errors
11.2.3 Software errors
The software operating manual contains a description of software error messages.
Symptom/message Cause Remedy
The dispensing tool
dispenses
incorrectly. • The sealing rings on the dispensing
tool are broken.
 Replace the sealing rings on the
dispensing tool.
Dispensing volume
too high. During retraction, the opening of the
pipette tip is in the liquid in the source
vessel. There are no air bubbles on the
lower end of the pipette tip when liquids
are being transported.
• The actual liquid volume in the source
vessel is higher than the entered
volume.
 Use the optical sensor to determine
the volume.
 Enter the correct volume.
 Avoid plates with very different well
filling heights.
• Liquid refilled after the application
was started.
 Do not refill liquid after the
application has started.
• Incorrect labware used.  Use the labware that was selected in
the application software.
Dispensing volume
too low. The pipette tip draws in air. There are air
bubbles in the pipette tip.
• The actual liquid volume in the source
vessel is lower than the entered
volume.
 Use the optical sensor to determine
the volume.
 Enter the correct volume.
 Avoid plates with very different well
filling heights.
• Incorrect labware used.  Use the labware that was selected in
the application software.
Residual liquid in
pipette tip. • Incorrect volume specification in the
destination vessel.
• After dispensing, the liquid will be
withdrawn from the destination vessel
to the pipette tip.
 Use the optical sensor to determine
the volume.
 Enter the correct volume.
 Avoid plates with very different well
filling heights.
• Incorrect liquid class selected.
• Inappropriate dispensing parameter.  Select the liquid class that matches
the liquid.
 Optimizing dispensing parameters
(refer to epMotion software operating
manual).
• Liquid wets the pipette tip.
• Liquid tends to foam.  For several dispensing steps,
exchange the pipette tip more often.
 Optimizing dispensing parameters
(refer to epMotion software operating
manual).