C.B.S. Scientific TTGEK-2401-220 - Manual (Page 39)

TTGE Instruction Manual, version 8/30/2011
39
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3.6 Gel Casting Techniques- continued
C. Vertical gradient gel casting using gradient maker (GM-40) and a mini-pump for DGGE.
1. Alternatively, you may choose to use a “mini-pump” or other peristaltic pump to cast gels as
shown in (Fig 3-5). If so, secure the gradient maker to a ring stand and connect the outlet
tubing to the mini-pump tubing adapter. Connect tubing from mini-pump to a 20ga. needle for
affixing between glass plates.
Fig. 3-5
2. For TTGE and SSCP single percentage gels, replace the gradient maker with flask (more
consistent than syringe).
Refer to section 3.7 for Gradient Gel Formula Protocol.
See section 4.3 for running conditions.