C.B.S. Scientific TTGEK-2401-220 User Manual

Page 46

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46

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3.10 Removing the Gels

1. When run is completed, turn off power supply, disconnect recirculating tubes and power

leads, remove cassettes from tank and unclamp glass sandwiches from cassettes.

Remove Gel Wrap™ by pulling away from plate.

2. Using a wedge plate separator,cat. # WPS-100, pry plates apart and immerse gel/plate

in buffer tray. Stain with EtBr (0.5g/ml) for 5-10 minutes. Lift gel on plate out of tray and

rinse with ddH20. Flip over onto Saran® wrap and peal off glass plate. View bands on

transilluminator. Electro-Blot or photograph.

3. If using borosilicate glass plates, scan EtBr stained bands directly through glass without

disassembly of gel plate sandwich.

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