C.B.S. Scientific TTGEK-2401-220 - Manual (Page 40)

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separation sheet
notched glass plate
spacer
unnotched glass plate
shim sheet
front panel with
captive thumbscrews
casting chamber
lid (used to tilt
assembly)
separation sheet
figure 1.
D. Vertical gradient gel casting using GM-40 gradient maker, Mini-Pump and Multi-Gel Caster for DGGE.
1) Multi-Gel Caster Assembly
a) Clean the gel caster with soap and water and the glass plates with alcohol.
b) Place lid on bench and put the top end of the casting chamber (locating groove provided) on
the lid so that it rests at an angle with the gasket facing up.
c) Start by stacking one separation sheet, followed by unnotched glass plate, spacer set, then
notched glass plate. Repeat this sequence until all 4 sets (or 10 sets) are neatly stacked into the
casting chamber. Place one additional separation sheet on the last glass plate. See figure 1.
d) Place front panel on chamber and tighten the 5 captive thumbscrews. With 4 (or 10) sets of
glass plates with 0.75mm spacers, the front panel seals against the gasket and compresses
the glass sandwiches. Using fewer than 4 (or 10) glass plate sets at 0.75mm thick will require
the use of the enclosed shim sheets to achieve a tight seal and light compression of the glass
plate sets. Use of other than 0.75mm thickness spacers may require the use of additional shim
sheets, which must be ordered separately.
e) Bring assembled caster to the vertical position. Test the tightness of the assembly by pulling
one of the polycarbonate shim sheets. If it comes out easily, use extra shims to increase
tightness against the front panel.