C.B.S. Scientific TTGEK-2401-220 - Manual (Page 41)

TTGE Instruction Manual, version 8/30/2011
41
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f) Set up components as shown below in figure 3-6.
Mini-Pump
Magnetic Stirrer
Gradient Maker Multi-Gel Caster
Stopcock
SC-2001
Silicone Tubing
SCT-100
g) For TTGE and SSCP single percentage gels, replace the gradient maker with flask.
h) For gradient gels connect a gradient former to a small peristaltic pump (MPP-100), and the
pump outlet to the luer valve mounted on the front panel. Fill caster to within 0.5cm of the glass
plate notches, turn pump off then close luer valve.
i) Carefully insert combs into glass plate sandwiches. Place lid on top of chamber until gels
polymerize.
Refer to section 3.7 for Gradient Gel Formula Protocol.
See section 4.3 for running conditions.
2) Disassembly
a) Remove lid and carefully remove one comb to ensure polymerization is complete. Return
caster to horizontal angle position as shown in figure 1. Disassemble the unit by unscrewing
thumbscrews. Release each gel sandwich by pulling the polycarbonate separation sheet.
If you are planning to store some of the gels for later use, leave the comb in place, rinse
excess acrylamide from glass plates, and wrap in Saran wrap or store in Zip-Lock bags in the
refrigerator.
b) Wash all Multi-Caster components after each use with mild detergent and rinse with DI water.
Fig. 3-6