Leica Biosystems CM1850 UV - Manual (Page 31)

31 Leica CM1850 – Cryostat
7.5.3 Manual defrosting of the quick freeze shelf
The quick freeze shelf may become hot during defrosting!
Therefore, do not touch it!
If increased frost formation occurs on the quick freeze shelf, especially af-
ter spray disinfection, a manual defrost cycle should be started (see 6.2.5),
which can be terminated when required.
7.6 Terminating work
7.6.1 Terminating daily work
• Lock the handwheel.
• Take the knife out of the knife holder and put it back into the knife box in
the cryochamber.
• Remove frozen section waste with a cold brush.
• Empty the section waste tray.
• Clean the storage shelves and brush shelf.
Only common detergents and disinfectants that contain 95 - 98 %
alcohol should be used for cleaning.
All components removed from the cold environment will collect
condensation. Therefore, dry them thoroughly before placing them
back into the cryochamber.
• Remove all specimens from the cryostat.
• Close the sliding window.
• Turn out the cryochamber illumination.
• Lock control panel 1 (Fig. 8) with the KEY button.
• Do not turn the instrument off with the automatic mains fuse as there
would be no cooling.
7. Daily use of the instrument