Leica Biosystems CM1850 UV - Manual (Page 5)

5 Leica CM1850 – Cryostat
Table of contents
7. Daily use of the instrument ................................................................................................................. 26
7.1 Specimen freezing ...................................................................................................................................... 26
7.1.1 Quick freeze shelf ........................................................................................................................................ 26
7.1.2 Stationary heat extractor ........................................................................................................................... 26
7.2 Specimen discs ........................................................................................................................................... 27
7.2.1 Inserting the specimen discs in the specimen head ............................................................................ 27
7.2.2 Specimen orientation ................................................................................................................................. 27
7.3 Sectioning ..................................................................................................................................................... 28
7.3.1 Trimming the specimen .............................................................................................................................. 28
7.3.2 Section thickness setting ........................................................................................................................... 28
7.4 Temperaturew selection chart (in minus °C) ........................................................................................ 29
7.5 Defrosting ..................................................................................................................................................... 30
7.5.1 Automatic defrosting of the cryochamber ............................................................................................. 30
7.5.2 Manual defrosting of the cryochamber .................................................................................................. 30
7.5.3 Manual defrosting of the quick freeze shelf .......................................................................................... 31
7.6 Terminating work ........................................................................................................................................ 31
7.6.1 Terminating daily work ............................................................................................................................... 31
7.6.2 Shutdown for a longer period ................................................................................................................... 32
8. Troubleshooting .................................................................................................................................... 33
8.1 Error messages in the display ................................................................................................................... 33
8.2 Temperature control button ...................................................................................................................... 33
8.3 Possible causes and remedies ................................................................................................................. 34
9. Cleaning, disinfection, maintenance ................................................................................................ 39
9.1 Cleaning ........................................................................................................................................................ 39
9.2 Spray disinfection with Leica Cryofect ................................................................................................... 40
9.3 Maintenance ................................................................................................................................................ 41
9.3.1 General maintenance ................................................................................................................................. 41
9.3.2 Removal of the microtome ......................................................................................................................... 42
9.3.3 Removal of the microtome cover ............................................................................................................. 44
9.3.4 Reinstallation of the microtome ................................................................................................................ 44
9.3.5 Replacement of the fuses .......................................................................................................................... 46
9.3.6 Replacement of the lamp ........................................................................................................................... 46
10. Ordering information, optional accessories .................................................................................... 48
10.1 Ordering information ................................................................................................................................... 48
10.2 Optional accessories .................................................................................................................................. 50
10.2.1 Mobile heat extractor ................................................................................................................................. 50
10.2.2 Thermal block .............................................................................................................................................. 50
11. Warranty and service .......................................................................................................................... 51
12. Decontamination Certificate (master) ........................................................................................................... 52