Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 2)

Table of Contents
Section 1 Preparation ..........................................1
1.1 Introduction ............................................................1
1.2 Method Overview ..................................................1
1.3 Immun-Star Products ............................................2
1.4 Complementary Products ......................................3
1.5 Storage and Stability of Kit Components ................4
1.6 Safety Instructions ..................................................6
Section 2 Assay Instructions ................................6
2.1 Experimental Strategy
and General Recommendations ............................6
2.2 Reagents Flowchart................................................11
2.3 Working Solutions ..................................................12
2.4 Assay Procedure ....................................................15
Section 3 Troubleshooting ..................................19
3.1 Troubleshooting Guide ..........................................19
References and Acknowledgements ......................26
Note on Electrophoresis and Blotting
Equipment..............................................................27
Appendix 1 Total Protein Detection Procedure ......28
* Note important blocking solution instructions on
page 13. High concentrations of blocker can lead to
high background on blots.