Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 22)

Section 3
Troubleshooting
3.1 Troubleshooting Guide
Probable Recommended
Problem Cause Solution
1. No reaction or weak a. Exposure time was i. Increase the
signal. too short. exposure time.
b. Immun-Star enhancer i. Use the
was not used with Immun-Star
nitrocellulose enhancer assay
membrane. protocol.
Exposure times
will vary; for
example, a
nitrocellulose
blot without
enhancer would
require a much
longer exposure
time to generate
a signal
comparable to
that generated
with enhancer in
5 min.
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