Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 28)

Troubleshooting Guide (continued)
Probable Recommended
Problem Cause Solution
d. Wash stringency i. Tween 20 is
was insufficient. necessary in
wash steps to
reduce back-
ground. The
concentration
can be increased
up to 0.3% if
background
persists.
ii. Increase the
number and
length of washes.
e. Second antibody i. Use the recom-
conjugate was used mended dilution,
at an excessive con- or determine the
centration. optimal dilution
experimentally.
f. Contamination i. Refer to the
occurred during instrument
transfer. instruction
manual for rec-
ommendations.
g. Solutions and i. Autoclave or
buffers are sterile-filter
contaminated with ddH
2
O prior
alkaline to use in making
phosphatase. solutions.
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