Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 4)

Section 1
Preparation
1.1 Introduction
The Immun-Star chemiluminescent detection system is a
sensitive, nonisotopic method for immunodetection of
specific antigens immobilized on nitrocellulose or PVDF
membrane. The system uses the powerful CDP-Star
chemiluminescent substrate and enhancer, which is activated
by an alkaline phosphatase enzyme conjugate. Drawing on
Bio-Rad’s expertise in protein blotting, the substrate and
enhancer are incorporated into a sensitive and easy-to-use
western blotting detection protocol.
The Immun-Star anti-mouse and Immun-Star anti-rabbit
chemiluminescent detection kits provide enough reagents to
assay 2,500 cm
2 of membrane or approximately 50 mini blots
of ~50 cm 2 each. The blotting reagents pack provides all the
complementary buffer reagents needed for western blotting,
matched in quantity to the detection kits. We also offer the
Immun-Star Intro chemiluminescent kits, with enough of all
reagents needed for 2 large blots or 8 mini blots. The
Immun-Star assay kits are for laboratory use only.
1.2 Method Overview
The first step in western blotting is the transfer of antigen onto
a solid support membrane by one of several methods. The
transfer can be done electrophoretically, following separation
1