Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 27)

Troubleshooting Guide (continued)
Probable Recommended
Problem Cause Solution
2. High background. a. Exposure time was i. Decrease
too long. exposure time.
ii. The use of
Immun-Star
enhancer is
required when
using nitrocellu-
lose membranes.
This will allow
decreased film
exposure times.
b. TBS washes after i. The washes are
transfer were critical to reduce
omitted or insufficient. spotted or
blotchy
background
development.
c. Blocking was i. Increase the
insufficient. duration of the
blocking step
and/or the
concentration of
blocker used.
ii. Try a different
blocking reagent,
such as casein,
gelatin, or BSA.
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