Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 23)

Troubleshooting Guide (continued)
Probable Recommended
Problem Cause Solution
c. Blot was allowed to i. Use heat-
dry after incubation sealable
with the chemilumi- bags to prevent
nescent substrate. drying of the
membrane.
d. Chemiluminescent i. Store the
substrate solution is reagent
inactive. at the proper
temperature,
4°C.
To test activity of
substrate, mix
1 ml of substrate
with 1–2 µl of
undiluted
conjugate and
look for light
emission in the
darkroom.
e. Primary antibody i. Store the anti-
solution is inactive or body solution at
nonsaturating. the proper
temperature.
Avoid bacterial
contamination,
heat inactivation,
and repeated
freeze-thaw
cycles.
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