Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 31)

Appendix 1
Total Protein Detection Procedure
Required Reagents
Catalog # Description
170-6533 Avidin-AP, 1 ml
or
170-3554 Streptavidin-AP, 0.5 ml
170-6529 N-Hydroxysuccinimide Biotinate (NHS-Biotin), in
dimethylformamide, 75 mM, 4 ml
170-6435 TBS Buffer Solution, 10x, 1 L
161-0715 Tween 20, 100 ml
Additional Reagents Required
Sodium borate, 10-hydrate (Na
2
B
4
O
7
•10 H
2
O), ACS reagent
grade
Sodium chloride (NaCl), ACS reagent grade
Working Solutions
Borate-Tween (1x BT)
(0.05 M Na
2
B
4
O
7
•10 H
2
O, 0.5 M NaCl, 0.2% Tween 20,
pH 9.3)
Dissolve 38.14 g Na
2
B
4
O
7
•10 H
2
O and 58.44 g NaCl in 1.9 L
ddH
2
O. Add 4 ml Tween 20, then bring to a final volume of 2
L with ddH
2
O and mix. Label this solution “1x BT”.
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