Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 24)

Troubleshooting Guide (continued)
Probable Recommended
Problem Cause Solution
ii. Antibody titer
was too low.
Increase the
concentration of
the antibody
used in the
assay.
iii. Tween 20 may
affect the
reactivity of some
antibodies.
Eliminate Tween
20 from the
assay (except the
wash after the
blocking step).
f. Conjugate is inactive. i. Store the
conjugate at the
proper
temperature as
recommended in
Section 1.5.
Avoid repeated
freeze-thaw
cycles.
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