Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 9)

1.6 Safety Instructions
1. Read the entire instruction manual before beginning the
assay.
2. Wear gloves and protective clothing, such as a laboratory
coat and goggles, when preparing and working with the
solutions in the assay. The Immun-Star enhancer contains
diethanolamine, which can cause skin and eye irritation. In
case of contact, immediately flush the skin or eyes with
copious amounts of water for at least 15 min, and remove
contaminated clothing.
Note: See Material Data Safety Sheet on diethanolamine
for additional information.
3. Work in well-ventilated areas. Avoid inhalation of vapors
when handling solutions containing diethanolamine.
4. Do not mouth-pipet any solutions.
Section 2
Assay Instructions
2.1 Experimental Strategy and General
Recommendations
Temperature. All steps are performed at room temperature
(22–25°C) unless indicated otherwise in the instructions. If a
lower assay temperature is preferred, it is advisable to double
the incubation and wash times for each 10°C decrease in
temperature.
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