Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 34)

8. Blot development – remove the membrane and drain
excess liquid without letting the blot dry. Place on a piece
of Saran wrap on a flat, level surface. Pipet the substrate
solution onto the membrane. Incubate 5 min. Remove the
membrane, draining the excess liquid from the blot. Seal in
a heat-sealable bag or in Saran wrap. Do not allow the
membrane to dry because this will result in a loss of signal.
9. Film exposure – expose X-ray or instant film to the blot
at RT. Exposure time will depend on the type of
membrane used and on the protein concentration.
Optimal conditions should be determined for each
particular application. Develop the film according to the
manufacturer’s instructions.
For further information, including a complete troubleshoot-
ing guide, consult the Biotin-Blot total protein detection kit
manual.
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