Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 26)

Troubleshooting Guide (continued)
Probable Recommended
Problem Cause Solution
ii. Transfer of
protein onto the
membrane was
incomplete. Stain
gel to assure
transfer of
protein. Use
prestained
standards to
monitor transfer
efficiency.
Consult the
appropriate
instrument
manual for
proper proce-
dures and rec-
ommendations.
h. Primary antibody i. Loss of reactivity
is not specific or may have
does not recognize occurred during
denatured antigens electrophoretic
(common with transfer. Pretest
monoclonals). the reactivity of
the antibody
against the
antigen by a dot
blot.
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