Bio-Rad Immun-Star™ AP Chemiluminescence Kits - Manual (Page 25)

Troubleshooting Guide (continued)
Probable Recommended
Problem Cause Solution
ii. The concentra-
tion of the
conjugate was
nonsaturating.
Increase the
concentration of
the conjugate
used in the
assay.
iii. Conjugate may
be contaminated,
causing inactiva-
tion of the anti-
body or enzyme.
Tap water may
cause inactiva-
tion; use only
ddH
2
O to pre-
pare all solutions.
g. Little or no antigen i. Tween 20 may
is bound to the wash bound
membrane. antigen from
the membrane.
Eliminate
Tween 20 from
the assay (except
the wash after the
blocking step).
22