Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 18)

6. Conditioning step (S1) parameters cannot be
changed.
Select the time or volt-hours option for the
Voltage Ramping Step (S2).
Press the adjacent soft key to activate the
alphanumeric keypad and enter a time up to
99:00 hours:minutes, or volt-hours up to
99,999.
Default values for Step 2:
7 cm
4,000 V - 2 hrs.
11 cm 8,000 V - 2:30 hrs.
17 cm 10,000 V - 3 hrs.
Press the NEXT key to continue.
7. Select the time or volt-hours option for the Final
Focusing step (S3).
Enter a time up to 99:00 hours:minutes or up to
99,999 volt-hours. If the maximum voltage is
not reached in Step 2, the rapid voltage slope is
applied to reach the maximum voltage.
Default values for Step 3:
7 cm
4,000 V 20,000 vhours
11 cm 8,000 V 35,000 vhours
17 cm 10,000 V 60,000 vhours
Select yes or no to insert an optional Hold step
(S4). No is the default parameter.
Note: If a Hold step is programmed the voltage
will be maintained at 500 V until the run is
stopped by the operator. If a Hold Step is not
programmed the run will stop upon the
completion of the final focusing step (S3).
Press the NEXT key to continue.
8. The first line of this screen displays the pre-set
method selected.
The second line displays the current limit/gel
(50 µA).
Enter the # of gels (IPG strips) to be run: 1–24
for 7 cm gels, 1–12 for 17 cm gels. 1 is the
default value.
Press to start the run and refer to Section 8
for the program status screens.
15
S1 250 V 00:15
S2 HRS:MIN vhours >
S2 4000 V 2:00 >
NEXT >
S3 hrs:min VHOURS
S3 4000 V 2:00 >
S4 500V/HOLD yes NO >
NEXT >
METHOD: " METHOD NAME"
LIMIT/GEL: 50 µA
ENTER # OF GELS: 1 >
PRESS TO START