Bio-Rad PROTEAN® IEF Cell Accessories User Manual
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Section 1
Introduction
1.1 Overview
The PROTEAN IEF Cell performs the first dimension isoelectric focusing of two dimen-
sional electrophoretic protein analysis. The PROTEAN IEF Cell can accommodate up to
twenty-four 7 cm or twelve 17 cm Bio-Rad ReadyStrip™ IPG Strips for simultaneous iso-
electric focusing.
The PROTEAN IEF system is comprised of a 10,000 V power supply with a Peltier tem-
perature control platform, disposable 12 channel rehydration/equilibration trays, 12 channel
focusing tray assemblies, and accessories.
The PROTEAN IEF Cell is fully programmable from the front panel and can store up to
10 user-defined methods. Passive or active (50V) rehydration can be programmed as a
separate method, or as the first step of a method. Each method holds up to ten (10) steps
where each step specifies the voltage, the manner of voltage ramping and the duration of the
time segment. In addition to the user defined methods, the PROTEAN IEF Cell contains three
pre-programmed methods specifically designed as a starting point for new samples and novice
users to help determine optimal focusing conditions of ReadyStrip IPG Strips.
1.2 Features
The PROTEAN IEF Cell provides the following features
•
Peltier temperature controlled focusing platform
•
Maximum Voltage of 10,000 V
•
Set voltage and voltage ramping profile in each step
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Time or Volt hour control in each step
•
Integral or separate rehydration step
•
Programmable current limit
•
Simultaneous focusing of twenty-four 7 cm strips, or twelve 17 cm IPG strips
•
Three pre-programmed (pre-set) methods
•
Ten user-defined methods
•
Run data collection via RS 232 port and optional Thermal Printer
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