Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 46)

Catalog
Number
Product Description
165-4070 Forceps, 1 pair
165-4071 Electrode wicks, pre-cut, 4 mm x 20 mm, 500
165-4072 Cleaning brushes, 2
165-4080 Thermal Printer, with cable and power adaptor, 100 V
165-4082 Thermal Printer, 120 V
165-4085 Thermal Printer, 220 V
170-2412 Thermal Printer Paper, 10 rolls
163-2129 Mineral oil, 500 ml
ReadyStrip™ IPG Strips
163-2000 ReadyStrip IPG Strips pH 3-10, 7 cm, 12
163-2001 ReadyStrip IPG Strips pH 4-7, 7 cm, 12
163-2003 ReadyStrip IPG Strips pH 3-6, 7 cm, 12
163-2004 ReadyStrip IPG Strips pH 5-8, 7 cm, 12
163-2005 ReadyStrip IPG Strips pH 7-10, 7 cm, 12
163-2007 ReadyStrip IPG Strips pH 3-10, 17 cm, 12
163-2008 ReadyStrip IPG Strips pH 4-7, 17 cm, 12
163-2010 ReadyStrip IPG Strips pH 3-6, 17 cm, 12
163-2011 ReadyStrip IPG Strips pH 5-8, 17 cm, 12
163-2012 ReadyStrip IPG Strips pH 7-10, 17 cm, 12
Section 16
References
General 2D electrophoresis
1. O'Farrell P. H. (1975) High resolution two-dimensional electrophoresis of proteins., J.Biol.Chem.
250, 4007–4021.
General solubilization
2. Rabilloud, T. (1996) Solubilization of proteins for electrophoretic analyses. Electrophoresis, 17,
813–829.
3. Link A.J.,Ed.(1999). "2-D Proteome Analysis Protocols." Methods in Molecular Biology, 112.
Humana Press, Totowa, NJ.
Thiourea /TBP
4. Molloy M. P. et.al. (1998) Extraction of membrane proteins by differential solubilization for sep-
aration using two-dimensional gel electrophoresis. Electrophoresis, 19, 837–844.
5. Herbert, B. R. et.al. (1998) Improved protein solubility in two-dimensional electrophoresis using
tributyl phosphine as reducing agent. Electrophoresis, 19, 845–851.
6. Rabilloud T. (1998) Use of thiourea to increase the solubility of membrane proteins in two
dimensional electrophoresis. Electrophoresis, 19, 758–760.
7. Ruegg, U. T. and Rudinger, J. (1977) Reductive cleavage of cystine disulfides with
tributylphosphine, Methods Enzymol., 47, 11–116.
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