Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 30)

8.2 Program Status Screen Instructions
1. Start Run Screen
Enter the number of IPG strips to run.
Enter a current limit up to 99 µA.
Enter the focusing temperature (10–25 °C).
Press to start the run.
2. Active/Passive Rehydration Display.
• The actual and set temperatures are
displayed (actual/set).
• Time is listed as elapsed time/programmed
time.
Press to stop the rehydration step.
3. When cell cover is opened the method is
paused. Close the lid to resume the rehydration
step.
4. Rehydration Complete Display - This screen is
displayed when the rehydration step is complete
and a pause after rehydration step has been
programmed. In the pause mode the system
maintains the programmed temperature. At this
point IPG strips can be transferred to the focusing
trays if needed, electrode wicks can be inserted
and sample and/or mineral oil or similar overlay
material can be added to the individual chan-
nels.
Press to start step 1 of the method.
5. S#/# (Current Step) Display
• The actual voltage and the total current are
displayed.
• The actual / set temperatures and the
current limit per gel are displayed.
• The Total VH listed are cumulative but do
not include the rehydration step (active) or
the optional final hold step.
• Time or vhours is listed as elapsed time
(vhours)/programmed time (vhours).
• The Method Name is displayed. "Run in
progress" is scrolled during the run.
27
REHYDRATION COMPLETE.
PRESS TO START
STEP #01 OF METHOD
** CELL COVER OPEN **
REHYDRATION PAUSED
CLOSE COVER TO PROCEED
ENTER # OF GELS: 1 >
µA LIMIT/GEL: 50 >
FOCUS TEMP: 20 °C >
PRESS TO START
REHYDRATION: AT 50 V
TEMP: 20/20 °C
TIME: 00:00/00:00
METHOD WILL START (OR PAUSE)
WHEN REHYDRATION COMPLETES
(Scrolled)
S 01/# XXV XXµA
20/20 °C XXµA/GEL
VH: 000000 00:00/XX:XX
NAME RUN IN PROGRESS
(Scrolled message during
run)