Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 42)

Problem Cause Solution
Burning of strips • Current limit too high. • We recommend a current limit
(this will cause large 50 µA/strip. Make sure the
fluctuations in correct number of IPG strips
resistance resulting was entered.
in the resistance error • IPG strips have dried out. • Make sure IPG strips are
message.) covered with mineral oil or
equivalent to prevent drying
out
• Electrode wicks too wet • Make sure the electrode wicks
or contain incorrect contain de-ionized water only
electrode solution. and are damp, not wet.
• Rehydration buffer • Check rehydration buffer
composition incorrect, concentration. Remake buffer if
salt concentration too high. if necessary.
Section 12
Accessories
12.1 Optional Thermal Printer
Thermal Printer can be directly used with the PROTEAN IEF Cell and comes ready to
connect to the RS232 serial port. A straight, serial cable is included with the printer and the
DIP switches are pre-set to match the PROTEAN IEF Cell requirements.
Configuration
The RS232 serial port Signal and Direction Thermal Printer
Pin 2 Transmit Pin 2
Pin 3 Receive Pin 3
Pin 5 Ground Pin 5
other pins Not connected other pins
Thermal Printer
1. Insert the DC plug on the AC adaptor into the power supply jack on the printer.
2. Plug the AC adaptor into an outlet.
3. Connect the straight, serial cable to the RS232 port of the PROTEAN IEF Cell and to
the serial port located on the back of the printer.
4. Load the thermal paper into the printer (refer to the Printer Manual).
5. The printer DIP switches have been preset to match the requirements of the PROTEAN
IEF Cell.
To print the current DIP switch settings proceed as follows:
a. Slide the power switch to ON while pressing the ONLINE button.
b. Release the ONLINE button and a list of the current settings will be printed. The
completed printout is followed by the prompt
"Continue?: Push On-line SW"
"Write?: Push paper feed SW"
c. Push the Feed button to return to ONLINE Mode.
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