Bio-Rad PROTEAN® IEF Cell Accessories User Manual
Page 26
6. The first line displays the step number, the
selected slope, and the entered voltage.
Select the time or volt-hours option for this step.
Enter a time up to 99:00 hours:minutes, or volt-
hours up to 99,999.
Press the NEXT key to continue.
7. The ADD(EDIT)/Insert/Delete screen with the
next step number in the method is displayed
Use the adjacent soft key to edit, insert, or
delete a step in the method
Press the NEXT key and repeat steps 5 and 6
to add steps to the method.
Select the method complete softkey when
programming is complete.
Note: An inserted step is inserted ahead of the
step # entered.
8. The save method screen is displayed after the
Method Complete key is selected. Select yes to
save the method to memory. Select no to run
the method without saving it to memory.
Press the NEXT key to continue.
9. The adjacent screen is displayed after saving
the method.
Enter the number of gels and edit current limit
per gel if needed to 99 µA per gel maximum.
Edit Focusing temperature if desired. Press
to start the run.
10. The Method Name Already Exists
If the method name already exists the adjacent
screen will be displayed.
Press the adjacent soft key to replace or rename
the method.
Press cancel save to return to the previous
screen.
11. Press the adjacent soft key to re-name the
method. When the cursor and enter symbols are
visible, use the alphanumeric keypad to enter
the new name.
Select the YES option to save the re-named
method. Select NO to continue to the run screen
without saving the method.
Press the NEXT key to continue to the run
screen.
12. The adjacent screen is displayed after replacing
the method. Enter the # of gels (IPG strips);
1–24 for 7 cm gels, 1–12 for 17 cm gels.
Press
to start the run and refer to Section 8
for program status screens.
23
S 01
xxxxxV
HRS:MIN
vhours
>
ENTER TIME:
00:00
>
NEXT
>
ADD
(EDIT) insert delete >
ENTER STEP #:
# >
NEXT
>
METHOD COMPLETE
>
"
METHOD NAME"
SAVE METHOD? YES
no
>
NEXT
>
METHOD NAME EXISTS!
REPLACE
>
RENAME
>
CANCEL SAVE
>
ENTER # OF GELS:
1 >
µA LIMIT/GEL:
50 >
FOCUS TEMP:
20 °C >
PRESS
TO START
ENTER # OF GELS:
1 >
µA LIMIT/GEL:
50 >
FOCUS TEMP:
20 °C >
PRESS
TO START
RENAME AND SAVE
>
"
METHOD NAME"
>
YES no
>
NEXT
>