Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 2)

Table of Contents
Page
Section 1 Introduction ....................................................................................................1
1.1 Overview ......................................................................................................................1
1.2 Features ........................................................................................................................1
1.3 Specifications................................................................................................................2
1.4 Unpacking ....................................................................................................................2
1.5 Safety ............................................................................................................................3
Section 2 Basic Set-Up and Operation..........................................................................4
2.1 Instrument Components and Controls..........................................................................4
2.2 Basic Operation ............................................................................................................7
2.2.1 Programming Parameters ................................................................................7
2.2.2 Programming Instructions................................................................................8
Section 3 Rehydration Program..................................................................................10
3.1 Rehydration Programming Diagram ..........................................................................10
3.2 Rehydration Programming Instructions ....................................................................11
Section 4 Running Preset Methods ............................................................................12
4.1 Preset Method Programming Diagram ......................................................................13
4.2 Preset Method Programming Instructions..................................................................14
Section 5 Stored Methods (RUN, EDIT, DELETE)..................................................16
5.1 Stored Method Programming Diagram......................................................................16
5.2 Stored Method Programming Instructions ................................................................17
Section 6 Programming a New Method......................................................................20
6.1 New Method Programming Diagram ........................................................................21
6.2 New Method Programming Instructions....................................................................22
Section 7 Editing a Method While Running (Real Time Editing) ..........................24
7.1 Real Time Editing Programming Diagram ................................................................24
7.2 Real Time Editing Programming Instructions ..........................................................25
Section 8 Method Status Screens ................................................................................26
8.1 Program Status Screen Diagram ................................................................................26
8.2 Program Status Screen Instructions............................................................................27
Section 9 Error Screens ................................................................................................29
Section 10 Reagent and Strip Preparation ..................................................................31
10.1 IPG Buffers and Reagents ..........................................................................................31
10.1.1 IPG Strip Rehydration Buffers....................................................................31
10.1.2 2nd Dimension Equilibration Buffers ........................................................33
10.2 IPG Strip Preparation and Handling ..........................................................................33
10.2.1 IPG Strip Rehydration ................................................................................33
10.2.2 Isoelectric Focusing ....................................................................................35
10.2.3 IPG Strip Storage ........................................................................................38
10.2.4 IPG Strip Equilibration (prior to 2nd dimension) ......................................38
Section 11 Troubleshooting ............................................................................................38
Section 12 Accessories ....................................................................................................39
12.1 Optional Thermal Printer Model DPU ......................................................................39
12.2 ReadyStrips IPG Strips ..............................................................................................41