Bio-Rad PROTEAN® IEF Cell Accessories User Manual

Page 34

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Error Display Screens

Description of Error Screens

10.

System error.
Recycle the power to reset the instrument. Turn
off the mains power, then wait 10 seconds
before turning the mains power back on.
If the problem persists, record the values listed
and call Bio-Rad at 1-800-4BIORAD or
contact your local Bio-Rad representative.

11.

System error.
Recycle the power to reset the instrument. Turn
off the mains power, then wait 10 seconds
before turning the mains power back on.
If the problem persists, record the values listed
and call Bio-Rad at 1-800-4BIORAD or
contact your local Bio-Rad representative.

12.

System error.
Recycle power to reset instrument. Turn off
the mains power, then wait 10 seconds before
turning the mains power back on.
If the problem persists, record the values listed
and call Bio-Rad at 1-800-4BIORAD or
contact your local Bio-Rad representative.

Section 10
Reagent and Strip Preparation

10.1 IPG Buffers and Reagents

10.1.1. IPG Strip Rehydration Buffers

Prior to isoelectric focusing, the IPG strips are rehydrated with rehydration buffer. Two
different rehydration buffers are listed; one for rehydration without sample and one for
sample application during rehydration. Rehydration with protein sample minimizes
solubility problems and allows for larger sample loads. The optimal rehydration buffer
composition for each type of sample is best determined empirically.

1,2,3

31

PROT. IEF E TRAP

ERR: XXX ERRV=XXX

T=XX, CTXT=XXX

CYCLE POWER TO RESET

PROT. IEF E TRAP

/0 ADDR=xxxx:xxxx

T=XX, CTXT=XXX

CYCLE POWER TO RESET

PROT. IEF E TRAP

ME: XXX, TYPE=XXX

T=XX, CTXT=XXX

CYCLE POWER TO RESET

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