Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 37)

Fig. 6. Rehydration/equilibration tray & focusing trays.
Open the IPG strip package and carefully remove the protective cover from the IPG
strip(s) with forceps. Discard the cover.
Fig. 7. Cover removal.
Add the appropriate rehydration solution (with or without protein sample) to each channel.
Bio-Rad ReadyStrip IPG Strip Volume per Channel
7 cm strip 125 µl
17 cm strip 300 µl
34
Focusing tray
Rehydration/equilibration tray