Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 25)

6.2 New Method Programming Instructions
1. Select the NEW METHOD mode to program
a new method.
2. Use the adjacent soft-key to activate the
alphanumeric keypad and enter a program
name with up to 10 characters.
Select YES or No for a rehydration step
(optional). NO is the default.
Enter a focusing temperature between 10–25 °C.
20 °C is the default value.
Press the NEXT key to continue.
Note: If a rehydration step is programmed
proceed to Step 3. If a rehydration step is not
programmed proceed to Step 5.
3. Select the Active (50 V) or Passive rehydration
option. Passive rehydration is the default.
Enter a rehydration temperature between
10–25 °C. Press the NEXT key to continue
4. Enter a rehydration time between 1 minute and
99 hours.
Select yes to insert a pause after the rehydra-
tion step. Select no to proceed immediately with
the first focusing step.
Press the NEXT key to continue.
Note: A pause is needed for inserting electrode
wicks, adding mineral oil, adding sample, or
transferring the strips from the disposable tray
to the running tray.
5. Use the adjacent soft-key to activate the
alphanumeric keypad and enter the desired
voltage value (50–10,000 V).
Select the voltage ramping slope for the step.
Brackets [ ] will surround the selected option.
Note: Rapid [ ] voltage ramping is limited
by the current. The Linear [ ] voltage ramping
option increases voltage linearly with respect to
time. The Slow [ ] voltage ramping option
increases voltage according to a delayed voltage
ramping algorithm.
Press the NEXT key to continue.
22
REHYDRATION >
PRESET METHOD >
STORED METHOD >
NEW METHOD >
NAME: " METHOD NAME" >
REHYDRATION: yes NO >
FOCUS TEMP: 20 °C >
NEXT >
REHYDRATION STEP
active@50 V PASSIVE >
ENTER TEMP: 20 °C >
NEXT >
ENTER TIME: 12:00 >
INSERT PAUSE AFTER
REHYDRATION? YES no >
NEXT >
S 01 0 V >
SELECT VOLTAGE SLOPE:
RAPID [ ] >
NEXT >